In vitro large screening of bioactive compounds of ...

2015-12-30 · The reduction of fungal growth was checked through in vitro screening tests on fungi grown on liquid culture medium in 96- or 24-well microtitration plates. The potential of fungal growth reduction was tested for five phenolic acids, 17 terpenic compounds and eight other substances targetting oxidative metabolic chain in mitochondria.

Metabolite profiling coupled with statistical analyses for ...

2010-4-1 · (2008). Metabolite profiling coupled with statistical analyses for potential high-throughput screening of quantitative resistance to fusarium head blight in wheat. Canadian Journal of Plant Pathology: Vol. 30, No. 1, pp. 24-36.

Screening Plant and Fungal Metabolites in Wheat, Maize …

2015-4-16 · Screening cereal crops for plant and fungal metabolites such as mycotoxins is an important step in ensuring food safety, guarding animals and humans from consuming harmful mycotoxins or undesirable plant …

(PDF) Screening of Antimicrobial and Antioxidant …

Screening of Antimicrobial and Antioxidant Secondary Metabolites from Endophytic Fungi Isolated from Wheat (Triticum Durum) April 2013 Journal of Plant Protection Research 53(2)

The efficiency of some bioagent fungi in reduction of ...

Abstract. Madhi QH, Abass MH, Matrood AAA. 2021. The efficiency of some bioagent fungi in reduction of wheat seed decay and seedling damping-off disease with heavy metals interaction. Biodiversitas 22: 3984-3993. Biological control is an ecofriendly efficient measurement for disease control and heavy metals reduction in soils. The use of bioagent fungi such as Trichoderma koningii and ...

Targeted Screening of Fungal and Plant Metabolites in ...

We describe a method that can be applied, for targeted screening of 21 fungal and plant metabolites with automated online sample cleanup utilizing a Thermo Scientific Transcend system coupled to a Thermo Scientific Exactive high-resolution mass …

Evaluation of LC

2020-1-14 · fungal metabolites. First, a list of 200 metabolites, potentially being present in food samples was established. Next, specific detection and identification criteria were defined, which are based on accurate mass, peak intensity and isotopologue ratio. Application of these criteria to the suspected metabolites from the list

Targeted Screening of Fungal and Plant Metabolites in ...

2017-9-6 · Targeted Screening of Fungal and Plant Metabolites in Wheat, Corn, and Animal Feed Using Automated Online Sample Preparation Coupled to Orbitrap LC-MS Ebru Ates, Michal Godula, and Klaus Mittendorf Food Safety Response Center, Dreieich, Germany Method 63747 1. Schematic of Method It is a big challenge to analyze all these toxins with a

Screening endophytic actinobacteria with potential ...

Screening endophytic actinobacteria with potential antifungal activity against Bipolaris sorokiniana and growth promotion of wheat seedlings Elisandra Minotto1,3, Luciana Pasqualini Milagre1, Cristina Spadari1, Thaisa Feltrin1, Ana Elisa Ballarini1, José Carlos Germani2, Sabrina Pinto Salamoni3 and Sueli Teresinha Van Der Sand1*

Screening of plant and fungal metabolites in wheat, maize ...

A wide range of plant and fungal metabolites can occur in cereals and feed but only a limited number of target compounds are sought. This screening method is using a database of over 600 metabolites to establish contamination profiles in food and feed. Extracts were injected directly into an automat …

Mass Spectrometry Identifies Plant and Fungal Metabolites ...

2013-11-5 · Ates, E. (2014) ''Screening of plant and fungal metabolites in wheat, maize, and animal feed using automated on-line clean-up coupled to high resolution mass spectrometry.'' Food Chemistry, 142, 276–284; Zachariasova, M.et al. (2010).

Frontiers | Mycotoxin Uptake in Wheat — Eavesdropping ...

Fusarium mycotoxins represent a major threat for cereal crops and food safety. While previous investigations have described plant biotransforming properties on mycotoxins or metabolic relapses of fungal infections in plants, so far, the potential consequences of radical exposure in healthy crops are mostly unknown. Therefore, we aimed at evaluating whether the exposure to mycotoxins ...

Screening of Various Metabolites in Six Barley Varieties ...

2019-11-6 · 1. Introduction. Barley (Hordeum vulgare L.) is the main malting and brewing cereal, grown globally anges in climate greatly influence the microbial diversity of cereals. Shifts of Fusarium species have already been reported by several authors across the globe [1,2,3] is known that certain Fusarium species are affected by the rising temperatures and can be considered as an indicator of ...

Antimicrobial and Phytochemical Screening of Endophytic ...

2021-9-11 · Fungal metabolites properties of crude extract of endophyte isolated from Pinus roxburghii plant shows that it has antimicrobial activity. This work requires further pharmacological screening for the isolation and identification of active compounds from the plant and its endophytic fungi.

Screening of plant and fungal metabolites in wheat, maize ...

2014-1-1 · Survey sample screening with database for the occurrence of fungal and plant metabolites. The data processing of survey samples for screening against the fungal and plant metabolite database allowed the determination of additional compounds in parallel to the 15 target compounds. The results are listed in Table 4. Compounds which have been ...

Screening of Inhibitory Activity against α-Amylase of ...

2017-6-4 · Uzbekistan. The obtained data showed that 100 fungal endophytes isolated from 16 local antidiabetic plants produce metabolites with potent inhibiting activity in vitro (60-82%) and are expected to be a source for therapeutic agents in treatment Keywords Endophytic fungi, α-amylase, Inhibitory activity, Bio-active natural products A ccepted:

screening and identification of the biological control ...

2018-10-4 · However, wheat yield and grain quality are greatly affected by disease epidemics and growing-season climates. Considerable losses occur due to plant diseases caused by soil-borne pathogenic fungi (Aggarwal et al. 2003). Bipolaris sorokiniana, a soil-borne fungal pathogen, is the causal agent of common root rot in wheat (Wei et al. 2017). The ...

The screening and identification of the biological control ...

Bipolaris sorokiniana is a soil-borne fungal pathogen causing common root rot in wheat, and is difficult to control through chemical and agricultural means. Chaetomium spp. have been documented as potential biological control agents for plant diseases. In this study, seven Chaetomium strains were sc …

Research Article GC-MS Analysis of Secondary …

2019-11-11 · 20 The concentration of these metabolites is highest in leaves followed by inflorescence, fruit, root, and stem.21 The isolation of endophytic fungi from medicinal plants followed by screening of bioactive compounds has become one of the main areas of endophytic research 22, 23, 24 as medicinal plants are known to harbour endophytic

Chemical synthesis of culmorin metabolites and their ...

plants may have the ability to at least partly inactivate and coun-teract fungal virulence factors using various mechanisms.24 Data regarding CUL metabolites formed by plants and their bio-logical significance are lacking. Since the most prominent and important metabolites are …

Selection and screening of fungal endophytes against …

2021-3-1 · Rojas et al. (2020) found that fungal endophytes from wheat plants are able to reduce FHB caused by F. graminearum. The objectives of this work were to obtain endophytic fungi from wheat tissues, and to screen them for their ability of to suppress the wheat pathogens, F. graminearum, M. majus, and W. circinata, first using dual cultures, and ...

Screening, identification, and colonization of fungal root ...

2020-9-10 · The goal of the present research was to ascertain the potential root endophytic fungi against Dematophora necatrix, Hartig, the causal pathogen of white root rot in apples; however, it has an expanded range of hosts across different fruit trees. Out of 36 endophytic fungi segregated from symptomless roots of apple, wheat, maize, marigold, cherry, and garlic plants, only 9 isolates showed ...

Evaluation of LC-high-resolution FT-Orbitrap MS for the ...

2018-6-17 · (2011). Evaluation of LC-high-resolution FT-Orbitrap MS for the quantification of selected mycotoxins and the simultaneous screening of fungal metabolites in food. Food Additives & Contaminants: Part A: Vol. 28, Applications of High Resolution Mass Spectrometry to …

The screening and identification of the biological control ...

2018-11-1 · The screening and identification of the biological control fungi Chaetomium spp. against wheat common root rot. Hai-Mei Yue Department of Plant Pathology and MOA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, 100193 Beijing, P. R. China.

Exploring the diversity of endophytic fungi and screening ...

2021-7-29 · The fungal diversity is associated with different tissues of the same host plant and is also dependent on its geographical distribution and climatic conditions . Nowadays, it is an emerging challenge to analyze the diversity of conglomerated fungal endophytes for the discovery of novel biomolecules producing species and their role in the ecosystem.

Metabolomics and Cheminformatics Analysis of …

2016-9-30 · Metabolites 2016, 6, 31 3 of 15 using publicly available data for these, and related, molecules. The interaction potential between plant metabolites and proteins in fungus is explored computationally using high throughput small molecule

Target screening of plant secondary metabolites in river ...

2020-10-23 · Plants produce a large variety of chemical compounds, which may be categorized as primary and secondary metabolites. Primary metabolites are necessary for growth and maintenance of cellular functions of the plant while secondary metabolites play an important role, for example as defence (against herbivores, microbes, viruses or competing plants) and signal compounds to attract …

Natural Occurrence of Fungi and Fungal Metabolites in ...

2004-11-10 · Fresh tomatoes, homegrown and from supermarkets, with developing fungal lesions were collected. Each lesion was sampled, and the resulting fungal cultures were identified morphologically and extracted for analyzes of secondary metabolites. The tomatoes were incubated at 25 °C for a week, extracted, and analyzed for fungal metabolites. Extracts from pure cultures were compared with …

Screening, Identification and Evaluation of Potential ...

2017-11-3 · Screening, Identification and Evaluation of Potential Biocontrol ... In contrast, endophytic fungi inmedicinal plants, especially in tropical regions, are stillpoorly explored though they could represent a source of valuable new and bioactive ... plants, characterization of novel metabolites, and other topics related to endophytic symbioses ...

Screening of plant and fungal metabolites in wheat, maize ...

Screening of plant and fungal metabolites in wheat, maize and animal feed using automated on-line clean-up coupled to high resolution mass spectrometry January 2014 Food Chemistry 142:276-84

Screening and Isolation of Anti-Nematodal Metabolites ...

Abstract. Several plants and fungi were tested in random screening of anti-nemato-dal metabolites against Bursaphelenchus xylophilus using an immersion test. Among 30 species of the terrestrial plants tested, Magnolia grandiflora, Michelia hedyos-perma, and Nerium indicum showed nematicidal activity. From leaf extracts of M. grandiflora, a new sesquiterpene, 4,5-epoxy-1(10)E, 11(13 ...